KMCRIC

Proteomic analysis of the effect of acupuncture on the suppression of kainic Acid-induced neuronal destruction in mouse hippocampus.

Bae CH, Kim DS, Jun YL, Kwon S, Park HJ, Hahm DH, Lee H, Kim ST.

Evid Based Complement Alternat Med.

2013

2013

436315.

10.1155/2013/436315.

23970931

http://www.ncbi.nlm.nih.gov/pubmed/23970931

Acupuncture
Kainic acid
Epilepsy
Seuzures
Animals
Disease Models, Animal
Mice
Electrophoresis, Gel, Two-Dimensional
glial fibrillary astrocytic protein, mouse
Astrocytes
Hippocampus
CA3 Region, Hippocampal

KMCRIC 비평 보기 +

Y

Kainic acid (KA) is a neurotoxin that induces epileptic seizures and excitotoxity in the hippocampus. Acupuncture is frequently used as an alternative therapy for epilepsy, and it has been known to protect hippocampal neurons against KA toxicity. Using proteomic analysis, we investigated protein expression changes in the hippocampus following acupuncture stimulation at HT8. Eight-week-old male C57BL/6 mice (20-25 g) received acupuncture treatment at HT8 acupoint bilaterally once a day for 3 days and were then administered KA (30 mg/kg) intraperitoneally. Twenty-four hours after KA injection, neuronal survival and astrocyte activation in the hippocampus were measured, and protein expression in the hippocampus was identified by 2-dimensional electrophoresis. Acupuncture stimulation at HT8 suppressed KA-induced neuronal death and astrocyte activation in the hippocampus. We identified the changes in the expression of 11 proteins by KA or acupuncture stimulation at HT8 and found that acupuncture stimulation at HT8 normalized the expression of dihydropyrimidinase-related protein 2 and upregulated the expression of transcriptional activator protein pur-alpha, serine/threonine-protein phosphatase 5 and T-complex protein 1 subunit alpha, which are related to the survival of neurons. These results suggest that acupuncture stimulation at HT8 changes protein expression profiles in the hippocampus in favor of neuronal survival in KA-treated mice.

영어